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Showing: 61 - 80 of 159 results

  11. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  12. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  13. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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  14. TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

    Plasmid
    #216152
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216152-plasmid-map-sequence-id-437762

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  15. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  16. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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  17. AiP15385: pAAV-AiE0873m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5385)

    Plasmid
    #230875
    Purpose
    AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230875-plasmid-map-sequence-id-459525

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  18. AiP15747: pAAV-AiE0779m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5747)

    Plasmid
    #230882
    Purpose
    AiE0779m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230882-plasmid-map-sequence-id-459397

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  19. AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

    Plasmid
    #230880
    Purpose
    AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230880-plasmid-map-sequence-id-469440

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  20. AiP15746: pAAV-AiE0780m-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5746)

    Plasmid
    #230881
    Purpose
    AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    230881-plasmid-map-sequence-id-459399

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Showing: 61 - 80 of 159 results