-
Plasmid#182858PurposeHetero-dimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mCherry2-mApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF820-recipient_U6-sgRNA-EF1a-mCherry2
Plasmid#211647PurposeU6-sgRNA-EF1a-mCherry2DepositorInsertGuide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEYFP(Q69K)-mEGFP
Plasmid#190751PurposeExpression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2, mEGFP, and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP
Plasmid#182853PurposeHetero-trimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF820-sgCIDE-1_U6-sgRNA-EF1a-mCherry2
Plasmid#211649PurposeU6-sgRNA-EF1a-mCherry2DepositorInsertsgCIDE-1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF820-sgCIDE-2_U6-sgRNA-EF1a-mCherry2
Plasmid#211650PurposeU6-sgRNA-EF1a-mCherry2DepositorInsertsgCIDE-2 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF820-sgNT-1_U6-sgRNA-EF1a-mCherry2
Plasmid#211648PurposeU6-sgRNA-EF1a-mCherry2DepositorInsertNon-targeting control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192236Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192244Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192238Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple
Plasmid#182854PurposeHetero-tetrmer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mCherry2-mEGFP-mApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2
Plasmid#211688PurposeU6-sgRNA-EFS-Cas9-P2A-mCherry2DepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2
Plasmid#211689PurposeU6-sgRNA-EFS-Cas9-P2A-mCherry2DepositorInsertSpyCas9 and sgNT-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF826-sgCIDE-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2
Plasmid#211690PurposeU6-sgRNA-EFS-Cas9-P2A-mCherry2DepositorInsertSpyCas9 and sgCIDE-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF826-sgCIDE-2_U6-sgRNA-EFS-Cas9-P2A-mCherry2
Plasmid#211691PurposeU6-sgRNA-EFS-Cas9-P2A-mCherry2DepositorInsertSpyCas9 and sgCIDE-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM8315
Plasmid#213988PurposePrpsM driven expression of mCherry2-LDepositorInsertmCherry2-L
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only