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pJBL3907

Plasmid

#193140

Purpose

Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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A0A0L6Z980

Plasmid

#163189

Purpose

Inducible expression of UniProt identifier A0A0L6Z980

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0L6Z980

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0F0C8R9

Plasmid

#163192

Purpose

Inducible expression of UniProt identifier A0A0F0C8R9

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0F0C8R9

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0U5NXR6

Plasmid

#163265

Purpose

Inducible expression of UniProt identifier A0A0U5NXR6

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U5NXR6

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LIP-WT

Plasmid

#16657

Depositor

Bert Vogelstein

Article

Cheong et al Science. 2006 Nov 24. 314(5803):1308-11.

Insert

Liposomase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
A0A0U5JSS4

Plasmid

#163183

Purpose

Inducible expression of UniProt identifier A0A0U5JSS4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0U5JSS4

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLNL1135

Plasmid

#160866

Purpose

PJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Chloramphenicol Acetyltransferase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PJ23110_BBa_B0029

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Neil Fairweather, Robert Fagan

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Tags

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRPF144

Plasmid

#106372

Purpose

C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

Depositor

Neil Fairweather, Robert Fagan

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Pcwp2-gusA (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Pcwp2

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSJ111

Plasmid

#78707

Purpose

Modular vector for constitutive secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pveg

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pDSW1728

Plasmid

#120812

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAP24

Plasmid

#70190

Purpose

Modular vector for secreted luciferase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

sLucopt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAF259

Plasmid

#105494

Purpose

pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

Insert

Bitlucopt

Use

Atc-dependent expression in c. difficile

Tags

Expression

Mutation

Promoter

Ptet

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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CcCDP_p15A

Plasmid

#179272

Purpose

Expresses CcCDP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Insert

cellodextrin phosphorylase

Use

Synthetic Biology

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7 lacO

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Neil Fairweather, Robert Fagan

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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