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LentiCRISPR v2-sgAMPK alpha1 clone1

Plasmid

#162119

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Human SHIP1 (clone hp51CN)

Plasmid

#123347

Purpose

Expression of human SHIP1

Depositor

Christophe Erneux

Article

Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

Insert

INPP5D (INPP5D Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha1 clone2

Plasmid

#162120

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HH06-LP Clone 6 heavy chain

Plasmid

#192176

Purpose

Clone 6 heavy chain (HH06)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HH02-LP Clone 2 heavy chain

Plasmid

#192175

Purpose

Clone 2 heavy chain (HH02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HK02-LP Clone 2 light chain

Plasmid

#192179

Purpose

Clone 2 light chain (HK02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Expression

Mutation

R661A+K1003H

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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D3 clone (p21/WAF1 promoter)

Plasmid

#16452

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgAMPK alpha1 clone3

Plasmid

#162121

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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XE40 pSP64T-PABP (Clone 63)

Plasmid

#17019

Depositor

Randall Moon

Article

Zelus et al Mol Cell Biol. 1989 Jun;9(6):2756-60. do

Insert

Poly(A) binding protein (pabpc1.S Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/shαSMA/GFP4_Seq1/clone1

Plasmid

#206138

Purpose

Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

alpha-SMA (Acta2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shαSMA/GFP4_Seq2/clone1

Plasmid

#201402

Purpose

Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Insert

alpha-SMA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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HH13 Clone 13 heavy chain

Plasmid

#192177

Purpose

Clone 13 heavy chain (HH013)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13 heavy chain (HH013)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWp63-clone3-12 (OptiHF-SpCas9)

Plasmid

#131737

Purpose

Expresses OptiHF-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

OptiHF-SpCas9

Use

Lentiviral

Tags

Flag

Expression

Mutation

Q695A+K848A+E923M+T924V+Q926A

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBS Matrilin-4 (clone 1_35)

Plasmid

#19192

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Matrilin-4 (clone 2_49)

Plasmid

#19218

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Matrilin 4 isoform 1 precursor (MATN4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
M35 Dpr yeast clone

Plasmid

#17191

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Tags

Gal4-AD

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only