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RCASBP-Y CHA

Plasmid

#11480

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Gateway ccdB reading frame cassette

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCHA1.1-Rap2a-V5

Plasmid

#149509

Purpose

Stable expression of human Rap2a.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

RAP2 (RAP2A Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA17

Plasmid

#48990

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

Ubiquitin10 promoter + 5' UTR

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-HT_RcCHAD

Plasmid

#127782

Purpose

Expresses RcCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

RcCHAD

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-3

Plasmid

#90327

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 6193956 and 6193957

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-OposCharlie2

Plasmid

#217169

Purpose

Mammalian expression vector for expression of OposCharlie2 transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

OposCharlie2 transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Chaplin1_DR

Plasmid

#217110

Purpose

Mammalian expression vector for expression of Chaplin1_DR transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Chaplin1_DR transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-OposCharlie2

Plasmid

#217098

Purpose

Donor plasmid of OposCharlie2 Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of OposCharlie2 transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Chaplin1_DR

Plasmid

#217040

Purpose

Donor plasmid of Chaplin1_DR Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Chaplin1_DR transposon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT

Plasmid

#214041

Purpose

Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase

Plasmid

#214043

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi

Plasmid

#214049

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-PCi

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ORF3cHA

Plasmid

#208653

Purpose

Express ORF3c-HA in mammalian cells

Depositor

Andrew Firth

Insert

ORF3c (ORF3a )

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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