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Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER2-LSSmGFP
Plasmid#186538PurposeLocalization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiencyDepositorInsertLSSmGFP
UseTagsCalreticulin and KDELExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Carl-YeNL-KDEL_pcDNA3
Plasmid#89516PurposeYellow color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertYeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Carl-CeNL-KDEL_pcDNA3
Plasmid#89509PurposeCyan color variant of bright luminescent protein, targeted to the endoplasmic reticulumDepositorInsertCeNL
UseTagsCalreticulin and KDELExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-ER
Plasmid#164166PurposeExpresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmCherry2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER2-hfYFP
Plasmid#186518PurposeLocalization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiencyDepositorInsertHyperfolder YFP
UseTagsCalreticulin and KDELExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorInsertCALR (CALR Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mWasabi-ER
Plasmid#164165PurposeExpresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmWasabi
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-ER-3
Plasmid#186960PurposeER lumen localizaing HaloTagDepositorInsertCALR (CALR Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP spGFP-ERGIC53
Plasmid#38270DepositorInsertERGIC-53 (LMAN1 Human)
UseRetroviralTagsEGFP with Calreticulin signal peptide at N-termin…ExpressionMammalianMutationdeleted signal peptide (1-30 amino acids)PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
actc1b- mKate2-KDEL-pA
Plasmid#109494PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequenceDepositorInsertactc1b- mKate2-KDEL-pA
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Calr-KDEL-IRES-Puromycin
Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
canxTM-mEmerald (cytosolic)
Plasmid#186962PurposeExpresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
UseTagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) and add ER signal…PromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-ER-EmEGFP
Plasmid#169000PurposeExpress ER-EmEGFP in zebrafishDepositorInsertER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
UseUnspecifiedTagsEGFPExpressionMutationPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_erGAP3
Plasmid#78118PurposeLow affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulumDepositorInserterGAP3
UseTagsKDEL and calreticulin signal peptideExpressionMammalianMutationS175G, D180Y, I167V and L15Q in GFP moiety and D…PromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorInsertRRBP1 (RRBP1 Human)
UseTagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNGVL4a-hCRTmE6mE7mL2
Plasmid#104153PurposeExpresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cellsDepositorUseTagsExpressionMammalianMutationresidues 11 to 200 of the late protein L2PromoterCMVAvailable sinceDec. 18, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits