Addgene

We narrowed to 1,087 results for: biotin

Showing: 61 - 80 of 1087 results

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pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-BioID2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-TurboID-3xFLAG

Plasmid

#186814

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

TurboID-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-TurboID-MCS

Plasmid

#186813

Purpose

CAG promoter-driven expression of TurboID-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-TurboID

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

BioID2-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Use

Tags

tagRFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Ruby2-NS

Plasmid

#166569

Purpose

Multisite Gateway middle entry clone encoding mRuby2. Parton lab clone DJA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Ruby2

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Use

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Mutation

Promoter

ADH1/yeast

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET30b_T7Sav

Plasmid

#138588

Purpose

T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

Depositor

Markus Jeschek

Article

Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

Insert

T7-tagged streptavidin variant with native C-terminus

Use

Synthetic Biology

Tags

T7-tag

Expression

Bacterial

Mutation

Promoter

PT7

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-EGFP

Plasmid

#163846

Purpose

In vitro transcription of TurboID-EGFP. Parton lab clone JRS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-P2A-TurboID-NS

Plasmid

#166568

Purpose

Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

mKate2-P2A-TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-miniTurboID-EGFP

Plasmid

#163847

Purpose

In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 and pBAD promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS-NLS-TurboID

Plasmid

#163849

Purpose

In vitro transcription of nls-TurboID. Parton lab clone JST

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

nls

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp-Egl-Trbo

Plasmid

#179993

Purpose

Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

Egl-Trbo

Use

Tags

TurboID

Expression

Insect

Mutation

Promoter

UAS-Gal4

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pME-BASU-NS

Plasmid

#166565

Purpose

Multisite Gateway middle entry clone encoding BASU. Parton lab clone JRO

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-dGBP

Plasmid

#163855

Purpose

Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

dVHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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