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Plasmid#157590PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCNTN5 (CNTN5 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CNTN5-Fc(DAPA)-AviTag-6xHis
Plasmid#157026PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCNTN5 (CNTN5 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLU_103
Plasmid#225785PurposeThis is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.DepositorInsertPtn5_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL1058
Plasmid#70686PurposeTn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.DepositorInsertTransposon Tn5-1058
UseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEZ-Frt-lox-DT
Plasmid#11736DepositorInsertpEZ-Frt-lox-DT
UseCre/Lox; /frtTagsExpressionMutationPromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINS-Neo
Plasmid#31390DepositorInsertaph
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pbacNeoR
Plasmid#26349DepositorInsertBacterial promoter::NeoR::bacterial 3p flank
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBacPuroR-NeoR
Plasmid#34921DepositorInsertsBacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-2
Plasmid#61564PurposeStandardized mini-Tn5 delivery plasmid, kanamycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-6
Plasmid#61566PurposeStandardized mini-Tn5 delivery plasmid, gentamicin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP4-7
Plasmid#79811PurposeModified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmidDepositorInsertChloramphenicol acetyltransferase
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-4
Plasmid#61565PurposeStandardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP4-8
Plasmid#79813PurposeModified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugationDepositorInsertGentamicin resistance gene
UseHelper bhr plasmid for mobilizing other plasmids …TagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP4-11
Plasmid#79812PurposeModified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmidDepositorInsertKanamycin cassette and SacB gene (sacB )
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterKmAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRT802
Plasmid#68113PurposeIntegration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
aph11
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RP4-6
Plasmid#80385PurposeHelper plasmid for mobilisation derived from RP4. Spectinomycin/Streptomycin resistantDepositorInsertSpectinomycin/Streptomycin
UseHelper bhr plasmid for mobilizing other plasmids …TagsnoneExpressionMutationPromoterNoneAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
yTREX
Plasmid#177306Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only