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CMV-TO-nTEVPhcZ-2A-ZhccTEVP

Plasmid

#116063

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPhcZ-2A-ZhccTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-nTEVPtvR-2A-KtvcTEVP

Plasmid

#116068

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPtvR-2A-KtvcTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-nTEVPtvhcZ-2A-ZhctvcTEVP

Plasmid

#116064

Purpose

Expresses an engineered TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

nTEVPtvhcZ-2A-ZhctvcTEVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Gal

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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CMV:β2Ar-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Plasmid

#135458

Purpose

Mammalian expression of SPARK TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

β2AR-hLOV-TEVcs(ENLYFQ/M)-GAL4-V5

Use

Lentiviral

Tags

Flag, V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTO-mCherry-Kir2.1-HA-tevs-RXR

Plasmid

#179704

Purpose

TEVP-inducible mCherry-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2

Plasmid

#179705

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-gfp-Kir2.1-HA-tevs-KKYL

Plasmid

#179706

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQC_HA-p115 TEV-Myc #77#407

Plasmid

#42127

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

inserted ENLYFQ b/w 886 and 887

Promoter

CMV

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_HA-p115-TEV-cleaved #957#382

Plasmid

#42107

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Tags

HA

Expression

Mammalian

Mutation

deleted 886 to end and added ENLYFQ

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-TEV-12His

Plasmid

#137032

Purpose

empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Type

Empty backbone

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-nTEVp

Plasmid

#179666

Purpose

Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

nTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-cTEVp

Plasmid

#179667

Purpose

Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

K65E

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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HIS_TEV_hsSYX3

Plasmid

#179290

Purpose

E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Use

Tags

His6 + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7lac

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2408-Tier1-PhCMV-TEVsite

Plasmid

#169503

Purpose

Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease cleavage site

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2405-Tier1-PhCMV-TEV

Plasmid

#169500

Purpose

Tier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-tevs-KKMP

Plasmid

#179634

Purpose

TEVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

Plasmid

#179633

Purpose

SEAP RELEASE construct with AAMP motif control

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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