Addgene

Showing: 61 - 80 of 222 results

Results per page:

pTetON-DHFRdd-SpvB; CMV-mCherry

Plasmid

#89463

Purpose

Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Use

Tags

E. coli DHFRdd destabilization domain and mCherry…

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101_TIMER

Plasmid

#103057

Purpose

low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Use

Tags

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pOD_003

Plasmid

#155362

Purpose

Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pWJB30

Plasmid

#126505

Purpose

Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

Depositor

Ute Romling

Article

Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

Insert

adrA-8xHis

Use

Tags

8xHis

Expression

Bacterial

Mutation

Promoter

PBAD30

Availability

Academic Institutions and Nonprofits only

Enlarge
pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Use

Tags

mNeonGreen

Expression

Bacterial

Mutation

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPV25

Plasmid

#20667

Depositor

Raphael Valdivia

Article

Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Type

Empty backbone

Use

Tags

GFP mut3

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
SopB-EGFP

Plasmid

#183655

Purpose

C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AN

Plasmid

#122716

Purpose

pSKAP plasmid containing the unique DNA barcode "AN" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AN

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AS

Plasmid

#122721

Purpose

pSKAP plasmid containing the unique DNA barcode "AS" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AT

Plasmid

#122722

Purpose

pSKAP plasmid containing the unique DNA barcode "AT" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
A0A0T9WB25

Plasmid

#163264

Purpose

Inducible expression of UniProt identifier A0A0T9WB25

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0T9WB25

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AA

Plasmid

#122703

Purpose

pSKAP plasmid containing the unique DNA barcode "AA" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AB

Plasmid

#122704

Purpose

pSKAP plasmid containing the unique DNA barcode "AB" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AC

Plasmid

#122705

Purpose

pSKAP plasmid containing the unique DNA barcode "AC" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AD

Plasmid

#122706

Purpose

pSKAP plasmid containing the unique DNA barcode "AD" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AE

Plasmid

#122707

Purpose

pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKAP_AF

Plasmid

#122708

Purpose

pSKAP plasmid containing the unique DNA barcode "AF" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Insert

Barcode AF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge