Showing: 61 - 80 of 576 results
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Plasmid#112154PurposeMammalian expression plasmid for SH3-5R in a vector expressing mCherry.DepositorInsertSH3-5R (NCK1 Human)
UseTagsExpressionMammalianMutationonly the second SH3 domain is expressed in this c…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV1/2
Plasmid#91322PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV1/2DepositorInsertSH3RF2-1/3-SV1/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
UseTags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorInsertSH3BP1 (SH3BP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SH3PXD2A-PX (1-126)
Plasmid#119115PurposeBacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)DepositorInsertSH3PXD2A-PX (1-126) (SH3PXD2A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#1
Plasmid#163400PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#2
Plasmid#163401PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ssh3 gRNA#3
Plasmid#163402PurposeCas9-mediated knockout of Ssh3 in mammalian cellsDepositorInsertSsh3 (Ssh3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH35
Plasmid#175234PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailabilityAcademic Institutions and Nonprofits only -
pT22i5uasH3En2SE4P3Che(#1212)
Plasmid#184098Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationchkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…Promoter5xUASAvailabilityAcademic Institutions and Nonprofits only -
pDONR221-sh3bgr
Plasmid#192727PurposeGateway entry vector encoding zebrafish sh3bgrDepositorInsertsh3bgr (zgc:100896 Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-1/3-SV2/2
Plasmid#91408PurposeProtein expression and purification of human SH3 domain construct SH3RF2-1/3-SV2/2DepositorInsertSH3RF2-1/3-SV2/2 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-1
Plasmid#160066PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ 3m shRNA-1
Plasmid#160067Purpose3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGGcTGaTTaCAACAAGGATTG (mutations shown in lower c…Promotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3+ shRNA-2
Plasmid#160068PurposeKnock Down SH3+ Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA1
Plasmid#160208PurposeKnock Down MQWSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3- 3m shRNA1
Plasmid#160209PurposeKnock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).DepositorInsertARHGEF9 (Arhgef9 Rat)
UseRNAiTagsExpressionMutationGATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…Promotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only -
CBSH3- shRNA2
Plasmid#160210PurposeKnock Down MTLSH3- Collybistin isoformsDepositorInsertARHGEF9 (Arhgef9 Human, Rat)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 576 results