Addgene

We narrowed to 74 results for: Plk1

Showing: 61 - 74 of 74 results

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GST-PBD

Plasmid

#60880

Purpose

Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653

Depositor

Trina Schroer

Article

Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

Insert

Polo-like kinase 1 (PLK1 BC01486, Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only
pENTR_Mm_CEP55(WT)

Plasmid

#136333

Purpose

Mouse CEP55 cDNA in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

Insert

CEP55 (Cep55 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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GST-PBD H538A/K540M

Plasmid

#60879

Purpose

Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

Depositor

Trina Schroer

Article

Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

Insert

Polo-like kinase 1 (PLK1 BC01486, Human)

Use

Tags

GST

Expression

Bacterial

Mutation

H538A/K540M

Promoter

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only
pLENTI_Hu_ECT2(WT)

Plasmid

#136330

Purpose

Lentiviral expression of human ECT2 WT

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T327D

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(WT)

Plasmid

#136327

Purpose

Mouse MyoGEF-WT in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(T575A)

Plasmid

#136328

Purpose

Mouse MyoGEF-T575A mutant in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

T575A

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-V5_Hu_p115-RhoGEF(WT)

Plasmid

#136335

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal V5

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TetO::EGFP-hMeikin(8A mut)

Plasmid

#174707

Purpose

Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Tags

EGFP

Expression

Mammalian

Mutation

S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AA315

Plasmid

#215951

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA310

Plasmid

#215950

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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