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pDIRE

Plasmid

#26745

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Insert

iCre expression cassette

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES2Cx32WTEGFP

Plasmid

#78150

Purpose

Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

Depositor

Mario Bortolozzi

Article

Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

Insert

Connexin 32 (GJB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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synIRES_RC25

Plasmid

#189970

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

synIRES_RC25

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2_EF1a_TetOn_IRES_mCherry

Plasmid

#204155

Purpose

Lentivirus backbone expressing rtTA linked to mCherry

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Tet-On 3G

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.PPT.SFFV.MCS.IRES.dTomato

Plasmid

#171174

Purpose

Lentiviral expression vector co-expressing dTomato

Depositor

Dirk Heckl

Insert

dTomato

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Ubc.Luc.IRES.Puro

Plasmid

#33307

Depositor

Linzhao Cheng

Article

Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.MCS.IRES.GFP

Plasmid

#169280

Purpose

Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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SFG.CNb30_opt.IRES.eGFP

Plasmid

#22493

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)

Use

Retroviral

Tags

Expression

Mutation

L124T; K125-LA-Ins

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK

Plasmid

#133080

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full length

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pmRFP_LAP2beta_IRES_puro2b

Plasmid

#21047

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

LAP2_beta (TMPO Rat)

Use

Tags

mRFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 14, 2009

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25H

Plasmid

#91145

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry_a_tubulin_IRES_puro2

Plasmid

#21043

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-H

Plasmid

#182281

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

Plasmid

#106120

Purpose

Lentiviral expression of ALDH7A1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ALDH7A1 (ALDH7A1 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

pmRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L

Plasmid

#182279

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDIRECT_21C

Plasmid

#91130

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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