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Plasmid#160723PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.DepositorInsertS2-cAMPr
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-GCaMP6f
Plasmid#160722PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-GCaMP6f
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-ExRaiAKAR
Plasmid#160724PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.DepositorInsertS3-ExRaiAKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S4-ExRaiCKAR
Plasmid#160725PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.DepositorInsertS4-ExRaiCKAR
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM005
Plasmid#170009PurposeEncodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PvirKAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM002
Plasmid#170006PurposeEncodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpmrDAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM003
Plasmid#170007PurposeEncodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PmgtCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM004
Plasmid#170008PurposeEncodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PugtLAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM006
Plasmid#170010PurposeEncodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpagCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM007
Plasmid#170011PurposeEncodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PslyBAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-196a.M3
Plasmid#220467PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7
Plasmid#220464PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7.M4
Plasmid#220465PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-196a
Plasmid#220466PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only