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Plasmid#76646Purpose3rd generation lentiviral gRNA plasmid targeting human TGFBR1DepositorInsertTGFBR1 (TGFBR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-D882N
Plasmid#114062Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-D882N (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-D882NPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795R
Plasmid#114060Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795R (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795RPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-3XFLAG-m-Lmbr1l
Plasmid#123593DepositorInsertlimb region 1 like (Lmbr1l Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HR1bR181A R185A-C (B3-B4)
Plasmid#211855PurposeCDS for mutant HR1b degron in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyTagsExpressionMutationR181A R185APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-N-L-HR1bR181A R185A (B4-B5)
Plasmid#211856PurposeCDS for mutant HR1b degron in domestication vectorDepositorInsertHR1b
UseSynthetic BiologyTagsExpressionMutationR181A R185APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-BR1
Plasmid#192816PurposeExpresses BR1 in bacteriaDepositorInsertBR1 (BRI1 Mustard Weed)
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_2 [pBBR1 ORI]
Plasmid#198919PurposeLevel 0 partDepositorInsertpBBR1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_24 [pBBR1-UP ORI]
Plasmid#198937PurposeLevel 0 partDepositorInsertpBBR1-UP ORI
UseSynthetic BiologyTagsExpressionMutationS100L (C299T)PromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAP-BUBR1-K795A
Plasmid#114061Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertBUBR1-K795A (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationC2823A and G2826A (siRNA-resistance), BUBR1-K795APromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TGFBR1 gRNA (BRDN0001146778)
Plasmid#76644Purpose3rd generation lentiviral gRNA plasmid targeting human TGFBR1DepositorInsertTGFBR1 (TGFBR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TGFBR1 gRNA (BRDN0001145243)
Plasmid#76645Purpose3rd generation lentiviral gRNA plasmid targeting human TGFBR1DepositorInsertTGFBR1 (TGFBR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
35S:5mARF17 pLBR19
Plasmid#12073DepositorInsert5mARF17 (ARF17 Mustard Weed)
UseTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6
Plasmid#180428PurposeGenome engineering of AgrobacteriumDepositorInsertRecETh1h2h3h4_agrob6
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-AID-BubR1
Plasmid#47330Purposefor plasmid integration using the Flp-In system. AID at N-terminalDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
UseTagsAID and GFPExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP-NBR1-dLIR
Plasmid#74204PurposeRetroviral expression of GFP-NBR1 with aa 727-738 deletionDepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationDeleted amino acids 727-738PromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP-NBR1-dUBA
Plasmid#74205PurposeRetroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codonDepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationChanged aa 877 to encode for premature stop codonPromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -