-
Plasmid#64214PurposemCherry fused between Cry2 and PGK-1 to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only
-
pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]
Plasmid#197599PurposePan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactiveDepositorUseTagsExpressionWormMutationD387A, E490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-PGL-1-mHP1alpha
Plasmid#181894PurposeSoluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CERK1 D441V-YFPc-1
Plasmid#102403Purposesplit YFP. Plant expression of CERK1 D441V-YFPc-1DepositorInsertCERK1 (CERK1 Mustard Weed)
UseTagsYFPcExpressionPlantMutationD441VPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6-SUMO-AtTPR1(1-209)
Plasmid#177858PurposeBacterial Expression of TPR1DepositorInsertTPR1 (TPR1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6-SUMO-AtTPR2(1-209)
Plasmid#177860PurposeBacterial Expression of TPR2DepositorInsertTPR2 (TPR2 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6- Sumo-AtTPL(1-209)
Plasmid#177856PurposeBacterial Expression of TPLDepositorInsertTPL (TPL Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(1)-CRY2-mCherry
Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1
Plasmid#64207PurposemCherry fused between Cry2 and RGS4(del 1-33) to study cell migrationDepositorUseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1
Plasmid#175814PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDV18 [punc-47::SNG-1::CRY2olig(535)]
Plasmid#197598PurposeExpression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN0035-1-FLAG-His
Plasmid#218030PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN0035-1
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00MN-1-FLAG-His
Plasmid#218041PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00MN-1
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN009T-1-FLAG-His
Plasmid#218068PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN009T-1
UseTags1xFLAG-10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV06 [punc-17::SNG-1::CRY2(535)]
Plasmid#197597PurposeExpression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegansDepositorUseTagsExpressionWormMutationE490GPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
MBP-CRY1-PHR
Plasmid#26035DepositorInsertCRY1 (CRY1 Mustard Weed)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217345PurposecrRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81DepositorInsertscrCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -