1. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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1. pLKO.1puro-shWillin-A

   Plasmid

   #40885

   Depositor

   Paul Reynolds

   Article

   Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

   Insert

   shWillin-A (FRMD6 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

2. T4 Lysozyme R96H

   Plasmid

   #18453

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R96H

   Promoter

   Available since

   Aug. 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

   Plasmid

   #128453

   Purpose

   lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUSB4_CCCC

   Plasmid

   #48453

   Depositor

   Stephen Ekker

   Article

   Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

   Insert

   RVD sequence: HD HD HD HD
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRF1-GW ymTurquoise2

   Plasmid

   #118453

   Purpose

   Overexpression of yeast codon-optimized mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307-DPP4-G418

   Plasmid

   #158453

   Purpose

   Constitutive lentiviral expression of human DPP4.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   DPP4 (DPP4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHACK-QF2-AgU6-mCherry

   Plasmid

   #184530

   Purpose

   HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

   Depositor

   Christopher Potter

   Article

   Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

   Insert

   QF2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Anopheles U6 promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TOPO CCSP

   Plasmid

   #68453

   Purpose

   TOPO construct expressing CCSP for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Clara Cell Secretory Protein Promoter
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTL392

   Plasmid

   #168453

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   OsaHIP33
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUAST YFP Rab18 WT

    Plasmid

    #58453

    Purpose

    expression of drosophila YFP Rab18

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. rAAV2/cMG

    Plasmid

    #184539

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-cMG capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, A589D; insertion o…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-mu24-ORF24-2xStrep

    Plasmid

    #138453

    Purpose

    Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    mu24-ORF24 (ORF24 MHV68/KSHV)
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    mu24 amino acids 1-191 fused to ORF24 amino acids…

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC

    Plasmid

    #148453

    Purpose

    Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsRck-QHTRtoAAAA-shRNAres (DDX6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-TetO-BATF2

    Plasmid

    #178453

    Purpose

    doxycycline-inducible expression of Human BATF2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    BATF2 (BATF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid

    #188453

    Purpose

    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223-ZNF449

    Plasmid

    #88453

    Purpose

    Donor Vector containing ZNF449 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF449 (ZNF449 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. JO-pH720-SARS-CoV-2-S-tail-WT

    Plasmid

    #218453

    Purpose

    Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
    

    Use

    Tags

    eXact

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLX311_TRIP13

    Plasmid

    #184538

    Purpose

    Constitutive expression of TRIP13

    Depositor

    Andrew Hong

    Article

    Mittal et al Commun Biol. 2024 Apr 8;7(1):426. doi: 1

    Insert

    TRIP13 (TRIP13 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRC8400-SARS-CoV-2-NTD-BA.2-AVI

    Plasmid

    #184532

    Purpose

    Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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