1. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. JARID2-KO-gRNA-2

   Plasmid

   #131327

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

2. JARID2-KO-gRNA-1

   Plasmid

   #131328

   Purpose

   gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   JARID2-KO-gRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

3. SKOR1-gRNA-N-del

   Plasmid

   #131331

   Purpose

   gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   SKOR1-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SKOR1-gRNA-C-del

   Plasmid

   #131332

   Purpose

   gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   SKOR1-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LMX1B-gRNA-N-del

   Plasmid

   #131333

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MTF2_KO_ex11_gRNA-4

   Plasmid

   #131334

   Purpose

   gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   MTF2_KO_ex11_gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HoxD del control us

   Plasmid

   #131335

   Purpose

   gRNA to delete control region near HoxD. Use with HoxD del control ds

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   CTRL-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HoxD del control ds

   Plasmid

   #131336

   Purpose

   gRNA to delete control region near HoxD. Use with HoxD del control us

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   CTRL-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

9. HoxD del us1

   Plasmid

   #131337

   Purpose

   gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EVX2-gRNA-N-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HoxD del ds1

    Plasmid

    #131338

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

12. pX458_Ruby

    Plasmid

    #110164

    Purpose

    Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

    Depositor

    Philip Hublitz

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and Ruby2

    Expression

    Mammalian

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

    Plasmid

    #138295

    Purpose

    gRNA for CRISPR/Cas9 knockout of human TRIM21

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    gRNA TRIM21 (TRIM21 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-V5-SBP-C1-HshnRNPA1_Z

    Plasmid

    #148138

    Purpose

    Mammalian Expression of HshnRNPA1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPA1 (HNRNPA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation I136V compared to the tra…

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75233

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75236

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75237

    Purpose

    CRISPR/Cas9 plasmid against human NFATc1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc1 (NFATC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge