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Addgene
Showing: 61 - 80 of 2907 results

  1. pFP.R376

    Plasmid
    #138246
    Purpose
    Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).Pfu-RIR1-1*.(TGL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Availability
    Academic Institutions and Nonprofits only
    138246-plasmid-map-sequence-id-296588

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  2. TFORF2376

    Plasmid
    #143365
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD1 (MBD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    143365-plasmid-map-sequence-id-400334

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  4. TFORF3376

    Plasmid
    #144852
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TOX (TOX Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    144852-plasmid-map-sequence-id-404429

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  8. pDD376

    Plasmid
    #137022
    Purpose
    SapTrap mKate2-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized mKate2
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    137022-plasmid-map-sequence-id-274081

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  9. TFORF1376

    Plasmid
    #142383
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF146 (ZNF146 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    142383-plasmid-map-sequence-id-397167

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  11. FB376

    Plasmid
    #209129
    Purpose
    Multipartite assembly of the TU for YFP expression with the TU for the auxotrophy marker pyr4 from T. reseei
    Depositor
    Jose Marcos
    Article
    Ropero-Pérez et al Curr Res Biotechnol 2024
    Insert
    PgpdA:yfp:TtrpC::Ppyr4:pyr4:Tpyr4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209129-plasmid-map-sequence-id-412660

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  12. pDG03769

    Plasmid
    #195996
    Purpose
    Nanobody Proj-Nano28 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- ProjNano28-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195996-plasmid-map-sequence-id-385940

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  20. pGBW-m4133767

    Plasmid
    #152206
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;TapTag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152206-plasmid-map-sequence-id-295218

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Showing: 61 - 80 of 2907 results