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Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro1GFP
Plasmid#82365PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro1GFP
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204CPromoterT7Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - C2 (UCSF)
Plasmid#169209PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Crimson/P2A-ICP8 (pSLIK1)
Plasmid#113856PurposeFor Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8DepositorInsertICP8
UseLentiviralTagsP2A/CrimsonExpressionMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R7
Plasmid#83309PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R7
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/F223R/S202KPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro4GFP
Plasmid#82368PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro4GFP
UseTags6xHISExpressionBacterialMutationC48S/S65T/Q80R/N149C/S202CPromoterT7Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF138
Plasmid#135923PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCitrine
UseTransposonTagsmCitrineExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-SweetTrac1
Plasmid#169083PurposeExpression of SweetTrac1 biosensor in yeastDepositorInsertSweetTrac1
UseTagsExpressionYeastMutationPromoterPMA1Available sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R10
Plasmid#83310PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R10
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/F223R/S202K/K41DPromoterT7Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG5-BMLF1
Plasmid#72636PurposeMammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulatorDepositorInsertBMLF1/BSLF2
UseTagsExpressionMammalianMutationQ157HPromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Crimson/P2A-NLS/HA-HumBeta (pSLIK2)
Plasmid#113857PurposeFor Doxycycline-inducible expression of Humanized Beta synaptase gene, with red fluorescent protein gene E2-Crimson-P2A-Humanized Beta synaptase geneDepositorInsertHumanized Beta
UseLentiviralTagsP2A/CrimsonExpressionMutation"humanized" Beta genePromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF161
Plasmid#135926PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mCerulean3
UseTransposonTagsmCerulean3ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iE
Plasmid#83312PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iE
UseTags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iE betwe…PromoterT5Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF163
Plasmid#135927PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).DepositorInsertTNFRSF9 fused to mNeptune2
UseTransposonTagsmNeptune2ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-mir302-8mer4x (pEZ185)
Plasmid#229059PurposeDual-luciferase reporter containing 4 copies of the 8mer site of human miR-302aDepositorInsertHuman miR-302a binding sites
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NLS-HA (pKW919)
Plasmid#229047PurposeMammalian expression of CasRx-NLS-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNLS and NLS-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI5-CasRx-NES-HA (pKW387)
Plasmid#229048PurposeMammalian expression of CasRx-NES-HADepositorInsertCasRx (RfxCas13d)
UseCRISPRTagsNES-HAExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only