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Showing: 61 - 80 of 53920 results

  4. pSG5-BMLF1

    Plasmid
    #72636
    Purpose
    Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator
    Depositor
    S. Diane Hayward
    Article
    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.
    Insert
    BMLF1/BSLF2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q157H
    Promoter
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pDual-eGFP(Stop66)

    Plasmid
    #63218
    Purpose
    Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Stop66)
    Use
    Lentiviral
    Tags
    T7 and his6
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pAF161

    Plasmid
    #135926
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCerulean3
    Use
    Transposon
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135926-plasmid-map-sequence-id-270079

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  10. pAF163

    Plasmid
    #135927
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mNeptune2
    Use
    Transposon
    Tags
    mNeptune2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135927-plasmid-map-sequence-id-270084

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  11. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  13. pAF125

    Plasmid
    #222373
    Purpose
    Recombinant human IgG antibody vector without variable regions. Contains mTagBFP marker.
    Depositor
    Andrew S. Flies
    Article
    Georgiev et al Vet Med Nauki. 1978;15(4):93-8.
    Insert
    IGHG (partial)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222373-plasmid-map-sequence-id-441916

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  16. pAF200

    Plasmid
    #222375
    Purpose
    Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins
    Depositor
    Andrew S. Flies
    Article
    Georgiev et al Vet Med Nauki. 1978;15(4):93-8.
    Insert
    IGHG
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    222375-plasmid-map-sequence-id-441923

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Showing: 61 - 80 of 53920 results