Skip to main content
Addgene
Showing: 121 - 143 of 143 results

  3. INS-2A-luciferase-2A-CD19-GFP

    Plasmid
    #159349
    Purpose
    Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Inserts
    Luciferase-2A-human_CD19-2A-GFP
    Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
    Use
    Ins locus knockin donor vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    159349-plasmid-map-sequence-id-313044

    Enlarge

  4. TFORF3042

    Plasmid
    #144518
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TAF9 (TAF9 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144518-plasmid-map-sequence-id-404232

    Enlarge

  11. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Tags
    Expression
    Mutation
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75024-plasmid-map-sequence-id-450679

    Enlarge

  13. pTT3-ecdCD19-ST3-His_withSUMO

    Plasmid
    #223219
    Purpose
    Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    extracellular domain of B-lymphocyte antigen CD19 (CD19 Human)
    Use
    Tags
    Histag, SUMO, and Spytag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    223219-plasmid-map-sequence-id-443124

    Enlarge

  14. pD649-HAsp-CD19-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156714
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD19 (CD19 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156714-plasmid-map-sequence-id-407030

    Enlarge

  17. 18065-M01-412

    Plasmid
    #225664
    Purpose
    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue
    Depositor
    Dominic Esposito
    Article
    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:
    Inserts
    dsCopGFP
    delCD19 (CD19 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    C-terminally truncated (aa 1-333 only)
    Promoter
    SORE6-mCMVp and WPRE-SV40p
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225664-plasmid-map-sequence-id-452346

    Enlarge

  18. 18065-M02-412

    Plasmid
    #225665
    Purpose
    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue
    Depositor
    Dominic Esposito
    Article
    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:
    Inserts
    dsmCherry
    delCD19 (CD19 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    C-terminally truncated (aa 1-333 only)
    Promoter
    SORE6-mCMVp and WPRE-SV40p
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    225665-plasmid-map-sequence-id-452347

    Enlarge

  19. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

    Enlarge

  20. pSYC-201

    Plasmid
    #178963
    Purpose
    Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Seok-Yong Choi
    Article
    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178963-plasmid-map-sequence-id-356608

    Enlarge

  23. pSYC-204

    Plasmid
    #178964
    Purpose
    Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Seok-Yong Choi
    Article
    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    R79C
    Promoter
    Zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178964-plasmid-map-sequence-id-356606

    Enlarge

Showing: 121 - 143 of 143 results