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Showing: 91 - 120 of 153 results

  4. Hygro-iDEST

    Plasmid
    #75339
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75339-plasmid-map-sequence-id-142552

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  5. pTRIPZ-Myr-PTEN

    Plasmid
    #206983
    Purpose
    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal
    Depositor
    Ming Chen
    Article
    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:
    Insert
    PTEN (PTEN Human)
    Use
    Lentiviral
    Tags
    N-terminal myristoylation site
    Expression
    Mammalian
    Mutation
    Promoter
    tet-on promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206983-plasmid-map-sequence-id-408428

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  6. Puro-iDEST

    Plasmid
    #75336
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    75336-plasmid-map-sequence-id-149881

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  10. pCW57-GFP-2A-Cas9

    Plasmid
    #170805
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide
    Depositor
    Morten Frodin
    Article
    Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170805-plasmid-map-sequence-id-338370

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  12. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Use
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  15. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190169
    Purpose
    Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.
    Depositor
    Anna Akhmanova
    Article
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    EB3N-VVDfast-mClover3-iLID
    Use
    Lentiviral
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mutation
    1-189 aa from EB3
    Promoter
    TRE
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190169-plasmid-map-sequence-id-373726

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  19. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid
    #136884
    Purpose
    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
    Depositor
    Moritz Mall
    Article
    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi
    Insert
    engrailed repressor domain (en Fly)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    136884-plasmid-map-sequence-id-272359

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  20. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  22. pLenti-nSpCas9-NoCBE

    Plasmid
    #209038
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209038-plasmid-map-sequence-id-413620

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  23. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  24. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  25. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  26. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

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  27. pLenti-nSpCas9-TadA8.20m

    Plasmid
    #209045
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8.20m-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209045-plasmid-map-sequence-id-413618

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  29. pNoROS-CAT

    Plasmid
    #170152
    Purpose
    Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation
    Depositor
    Dimitri Pestov
    Article
    Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.
    Inserts
    catalase (Cat Mouse)
    roGFP2
    Use
    Genome integration via sleeping beauty transposon…
    Tags
    FLAG tag and Nucleolar Localization Sequence (NoL…
    Expression
    Mammalian
    Mutation
    Promoter
    RPBSA and TRE promoter
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170152-plasmid-map-sequence-id-335808

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Showing: 91 - 120 of 153 results