Addgene

We narrowed to 254 results for: Cdc42

Showing: 91 - 120 of 254 results

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pTriEX-Antenna-GDI Cdc42

Plasmid

#101681

Purpose

"Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Cdc42

Use

Tags

6xHis and FRET Antenna (mCerulean-cpVenus)

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSuperGFP-cdc42-prenyl siRNA

Plasmid

#22715

Depositor

Alaa El-Husseini

Insert

cdc42 siRNA (Rac1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMX GFP cdc42 Q61L

Plasmid

#14568

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

cdc42 Q61L (CDC42 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR

Plasmid

#51389

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

Cdc42 3'UTR (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

Plasmid

#118622

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed ttgcttt to cggtaag (496 - 502 nt in 3…

Promoter

synapsin I (rat)

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-Cdc42-T17N

Plasmid

#214289

Purpose

Express dominant negative Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Dominant negative Cdc42 (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-T17N

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

Plasmid

#118614

Purpose

expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

tetON CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Use

Tags

GFP1-10

Expression

Yeast

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-Cdc42/N12

Plasmid

#214290

Purpose

Express split-Cdc42 fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Cdc42 fragment fused with FRB (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-Cdc42/13C-FKBP-MBP

Plasmid

#214291

Purpose

Express split-Cdc42 fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Cdc42 fragment fused with FKBP (CDC42 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

T7

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

Plasmid

#176121

Purpose

Constitutivley active, mitochondrial localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

CDC42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-CDC42-G12V-deltaCAAX

Plasmid

#176094

Purpose

Constitutively active, nuclear localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

CDC42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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213-petDuet1-GBD-LUMP--cdc42

Plasmid

#65973

Purpose

Dual expression of GBD-LUMP and cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Inserts

LUMP
cell division cycle 42 (CDC42 Human)

Use

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Mutation

constitutively active Cdc42 (Q61L)

Promoter

T7

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Use

Tags

GFP1-10

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

Plasmid

#221329

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)

Use

Retroviral

Tags

Expression

Mutation

G12V

Promoter

TRE3G

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

Plasmid

#118615

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

tetON CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

Plasmid

#165434

Purpose

Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

Use

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

Plasmid

#165436

Purpose

Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

Use

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Mutation

H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

Promoter

CMV

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

Plasmid

#51390

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

miR-29 binding site mutant (position 577-599, 997…

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
GFP-wGBD

Plasmid

#26734

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

GFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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mRFP-wGBD

Plasmid

#26733

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

mRFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

Available since

April 21, 2011

Availability

Academic Institutions and Nonprofits only
dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP-H246,249D

Plasmid

#12598

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Type

Empty backbone

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
WASp(CRIB)-HaloTag-WASp(CRIB)

Plasmid

#191452

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntr1a-wGBD-mTurquoise2-wGBD

Plasmid

#176136

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge