Addgene

We narrowed to 218 results for: 5078

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px459-H3f3a KO sgRNA

Plasmid

#186940

Purpose

H3f3a KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3a KO sgRNA (H3f3a Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
ETS2_pCSdest

Plasmid

#53875

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ETS2 (ETS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA4/TO-Flag-H3.3

Plasmid

#47980

Purpose

tetracycline-inducible expression of human Flag tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

CMV promoter with tetracycline operator

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-H3.3WT-FLAG/HA

Plasmid

#128740

Purpose

Expression of histone H3.3WT-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3 WT (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

Promoter

MSCV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLYS1-MICU1-Flag

Plasmid

#50058

Purpose

Expression of human MICU1 with Flag tag in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MICU1 (isoform 2) (MICU1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

*

Promoter

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

See note below

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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ETS2_pet28a

Plasmid

#131664

Purpose

Bacterial expression of ETS gene ETS2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETS2 (ETS2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pIND-hygro-HA-H3.3

Plasmid

#47981

Purpose

Ponasterone A-inducible expression of human HA tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

Minimal Heat Shock Promoter

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIpuro-H3.3K4M-FLAG/HA

Plasmid

#128742

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

CMV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDK480

Plasmid

#134197

Purpose

Mammalian expression of Flip in SKAP and IRES GFP

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

untagged AltSKAP (hardened to G5siRNA) (KNSTRN Human)

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ImpBeta1-mEosEM

Plasmid

#218933

Purpose

Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

Importin Beta (KPNB1 Human)

Use

Tags

6xHis and mEos4b (EosEM)

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Use

Tags

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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MICU-APEX2

Plasmid

#79057

Purpose

A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)

Use

Lentiviral

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV immearly promotor

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAG26

Plasmid

#226743

Purpose

Expresses a reporter for a mitochondrial intermembrane space protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MICU1 (targeting sequence) (MICU1 Human)

Use

Lentiviral

Tags

GFP1-10

Expression

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRA_Hormone-recep

Plasmid

#109908

Purpose

Protein expression and purification of ESRRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRA_Hormone-recep (ESRRA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-eGFP-6xHis

Plasmid

#130981

Purpose

Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-GFP-6xHis (Mapre1 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

EB1 (MAPRE1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-mCherry-6xHis

Plasmid

#130984

Purpose

Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-mCherry-6xHis (Mapre1 Mouse)

Use

Tags

mCherry-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNB1

Plasmid

#192301

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNB1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNB1 (KPNB1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-mEF

Plasmid

#31765

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

Mutated in both EF-hand domains: D231A, E242K, D4…

Promoter

Available since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
FN-Ets2

Plasmid

#44603

Depositor

Robert Oshima

Article

Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

Insert

Ets2 (Ets2 Mouse)

Use

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_KPNB1_HEAT

Plasmid

#110017

Purpose

Protein expression and purification of KPNB1_HEAT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KPNB1_HEAT (KPNB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-sh1-rORF

Plasmid

#31764

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

RNAi-resistant ORF (rORF). 8 synonymous changes i…

Promoter

None

Available since

May 4, 2012

Availability

Academic Institutions and Nonprofits only

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