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pLIC-EcTOP

Plasmid

#224463

Purpose

Expression of recombinant Escherichia coli topoisomerase I

Depositor

Yuk-Ching Tse-Dinh

Article

Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

Insert

Escherichia coli TopA

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-196a.M3

Plasmid

#220467

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a

Plasmid

#220470

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-196a

Plasmid

#220466

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-NU-MT-P2A

Plasmid

#214898

Purpose

Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-NU-MT-P2A

Use

Tags

none

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-ER-PO-P2A

Plasmid

#214894

Purpose

Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-ER-PO-P2A

Use

Tags

none

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-ER-PM-P2A

Plasmid

#214893

Purpose

Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

Depositor

Tito Calì

Insert

SPLICSFAST-ER-PM-P2A

Use

Tags

none

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYUBcLIC-GFP

Plasmid

#183509

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Use

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Mutation

Promoter

T7 lac

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1spliced

Plasmid

#80628

Purpose

expression of Xbp1 RB isoform in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Use

Tags

Expression

Insect

Mutation

RB isoform

Promoter

hsp70

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pLIC.B3.CT0478

Plasmid

#11526

Depositor

Sung-Hou Kim

Article

Kim et al Proteins. 2006 Feb 1. 62(2):322-8.

Insert

ScpB

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B4.SPy1128

Plasmid

#11531

Depositor

Sung-Hou Kim

Article

Xu et al Proteins. 2004 May 1. 55(2):479-81.

Insert

phosphotransacetylase

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B4.TM1717

Plasmid

#11532

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2004 Sep 7. 10

Insert

YjeQ GDP binding protein (TM1717 T. maritima)

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B4.BSU37660

Plasmid

#11535

Depositor

Sung-Hou Kim

Article

Xu et al J Struct Funct Genomics. 2005 Nov 9. ():

Insert

phosphotransacetylase

Use

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B2.MPN330

Plasmid

#11536

Depositor

Sung-Hou Kim

Article

Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

Insert

FAD synthetase

Use

Tags

HisTag, non-cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pCW-LIC-N8C_Fab3b

Plasmid

#203685

Purpose

Expresses N8C_Fab3b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge