Showing: 61 - 80 of 143383 results
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Plasmid#65612Purposeexpress soluble His-tagged zebrafish CSF3a/Gcsfa in E. coliDepositorInsertCsf3a (csf3a Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorInsertCsf3b (csf3b Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pFLIP38
Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
Degron-KI p53 donor vector
Plasmid#65619PurposeDonor vector to generate Degron-KI DD-p53DepositorInsertp53 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Degron-KI ezh2 donor vector
Plasmid#65620PurposeDonor vector to generate Degron-KI DD-EZH2DepositorInsertEZH2 left and right HR arms
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Degron-KI PIK3CA donor vector
Plasmid#65621PurposeDonor vector to generate Degron-KI DD-PIK3CADepositorInsertPIK3CA Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Degron-KI SF3B1 donor vector
Plasmid#65622PurposeDonor vector to generate Degron-KI DD-SF3B1DepositorInsertSF3B1 Degron-KI left and right HR arms
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1DepositorInsertattP
UseBxb1TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-tagRFP-NLS-HA-Bxb1
Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
UseTagsHA, SV40 NLS, and tagRFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-gRNA
Plasmid#65626PurposeEmpty vector for the expression U6 driven gRNADepositorInsertU6-gRNA
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro2 AXL
Plasmid#65627PurposeExpresses AXL on cistronic transcript with puro resistance in mammalian cellsDepositorInsertTyrosine-protein kinase receptor UFO (AXL Human)
UseTagsExpressionMammalianMutationSpurious PDPRPHR on C terminusPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pIK208
Plasmid#65630PurposesgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"DepositorInsertU6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIK127
Plasmid#65631PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.DepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIK137
Plasmid#65632PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragmentDepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
UseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailabilityAcademic Institutions and Nonprofits only -
LRG (Lenti_sgRNA_EFS_GFP)
Plasmid#65656PurposeLentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter-driven sgRNA expression and EFS promo…AvailabilityAcademic Institutions and Nonprofits only
Showing: 61 - 80 of 143383 results