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Showing: 31 - 40 of 357 results

  1. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid
    #131757
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 Sp/xCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131757-plasmid-map-sequence-id-314450

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  2. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid
    #131756
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 Sp/xCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131756-plasmid-map-sequence-id-314449

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  3. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid
    #131755
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 SaCas9 gRNA
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131755-plasmid-map-sequence-id-314448

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  4. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Use
    Tags
    Expression
    Mutation
    changed Asp at position 249 + 250 to Val
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60488-plasmid-map-sequence-id-96845

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  5. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166032-plasmid-map-sequence-id-338747

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  6. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

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  10. pCD/NL-BH∆1

    Plasmid
    #41791
    Purpose
    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev
    Depositor
    Jakob Reiser
    Article
    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13
    Insert
    HIV-1 Gag/Pol and RRE sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    41791-plasmid-map-sequence-id-171188

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Showing: 31 - 40 of 357 results