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Plasmid#206392PurposeExpresses the DNA binding domain of the ePOU with a 6xHis tag for protein purificationDepositorInsertePOU
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-B4GALT3mut
Plasmid#203602PurposeReporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.DepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLuciferaseTagsExpressionMammalianMutationSeed sequences in miR-27b target sequences were m…PromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-MAIP1mut
Plasmid#203604PurposeReporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequencesDepositorInsertmatrix AAA peptidase interacting protein 1 (MAIP1 Human)
UseLuciferaseTagsExpressionMammalianMutationmiR-27b target sequences in the 3'UTR was mu…PromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD22y/PfPRMT5-PTP
Plasmid#203160Purposefor tagging PTP to the 5' end of PfPRMT5 gene by single crossoverDepositorInsertPfPRMT5 fragment, PTP tag and pDT 3' UTR
UseTags3' UTR of P. berghei dihydrofolate reductase…ExpressionMammalianMutationPromoterNoAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD22Y/PfPRMT5 deletion
Plasmid#203161Purposefor disruption of PfPRMT5 geneDepositorInsertPfPRMT5 fragment
UseTags3 myc tags and 3' UTR of P. berghei dihydrof…ExpressionMammalianMutationPromoterNoAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATm2allL+A
Plasmid#203750PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm2(allL+A) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.2
Plasmid#201404PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet-Seq1.1
Plasmid#201401PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/Col1a1/GFP4_Seq 1.1
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-Col1a1/GFP4_Seq1.2
Plasmid#201403PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Myc-tagged PSK (K57A)
Plasmid#197150PurposeExpression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsMycExpressionMammalianMutationChanged K 57 to APromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-PSK2 (K57A)
Plasmid#197118PurposeExpression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cellsDepositorInsertTAOK1 (K57A) (TAOK1 Human)
UseTagsMycExpressionMammalianMutationChanged K 57 to APromoterSV40Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC
Plasmid#148475PurposeInsect Expression of DmNot1_1149-1376DepositorInsertDmNot1_1149-1376 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_AD
Plasmid#148503PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717_X
Plasmid#147982PurposeInsect Expression of DmNot1_1-1717DepositorInsertDmNot1_1-1717 (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X
Plasmid#147986PurposeInsect Expression of DmNot1_1-1717-K1537SG1562EQ1658ADepositorInsertDmNot1_1-1717-K1537SG1562EQ1658A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X
Plasmid#147987PurposeInsect Expression of DmNot1_1-1717-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-R1197AN1203AF1204A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X
Plasmid#147988PurposeInsect Expression of DmNot1_1-1717-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-RNFKGQtoAAASEA (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only