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Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX2-AS-long-FlagTag (pcDNA3.1+)
Plasmid#75203PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsFLAGExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE- TRE-DEST-CBioTAP
Plasmid#53569PurposeLentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK1(K46N)
Plasmid#22897DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseTags3xHAExpressionMammalianMutationchanged Lysine 46 to AsparginePromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx C35S
Plasmid#36947DepositorInsertThioredoxin-1 C35S mutation (TXN Human)
UseTagsHis tagExpressionBacterialMutationcys 35 to serinePromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
p7133 pHAGE-P-CMVt-N-HA-GAW-PRKAR2B
Plasmid#100160PurposeExpresses PRKAR2BDepositorInsertPRKAR2B (PRKAR2B Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - DDX3Y sgRNA 2
Plasmid#70657PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against DDX3Y
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
bactin:GFP-DCX
Plasmid#105961Purposetransgenesis, ubiquitous expressionDepositorInsertDCX (DCX Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-Venus-Emi1 (914)
Plasmid#39854DepositorInsertEmi1 (FBXO5 Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Tmem98-FLAG
Plasmid#124501PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136463
Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046933
Plasmid#145592PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk3
Plasmid#136697PurposeAcceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046901
Plasmid#145725PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTags6xHIS, ECFP, and YFPExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046789
Plasmid#145660PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 Orf3b
Plasmid#158373PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf3b
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCk1
Plasmid#136695PurposeAcceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I beta
Plasmid#22155DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only