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Plasmid#194974PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCav1 in pT3TS-Dest
Plasmid#194293PurposeIn vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZWDepositorInsertcaveolin (cav1.S Frog)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1a
Plasmid#194291PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXMDepositorInsertcavin1a (cavin1a Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-mDia1-GBD (1-305 aa) in pEGFPN1
Plasmid#187280PurposeExpress EGFP-mDia1 GTPase-binding domainDepositorInsertmDia1 (Diaph1 Mouse)
UseTagsEGFPExpressionMammalianMutationGBD (1-305aa)PromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Sa-iPGM-bio
Plasmid#179523PurposeExpresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertS. aureus iPGM bio-6His
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Mo-iPGM-bio
Plasmid#179614PurposeExpresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coliDepositorInsertM. orale iPGM bio-6His
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIS_TEV_hsSYX3
Plasmid#179290PurposeE. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7lacAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNN-GFP
Plasmid#163664PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSN-GFP
Plasmid#163665PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NNS-GFP
Plasmid#163666PurposeExpresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TNF…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
F9SS-GFP
Plasmid#163670PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
F9SF-GFP
Plasmid#163671PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
F9FS-GFP
Plasmid#163672PurposeExpresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the 9 amino acids from the …PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H90
Plasmid#170340PurposeCFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgZmat3.52.6/Cre
Plasmid#167854PurposeExpresses Cre recombinase and an sgRNA targeting Zmat3DepositorInsertsgRNA targeting Zmat3
UseCRISPR, Cre/Lox, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-MIOX
Plasmid#166680PurposeYeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
wtVP1 + VP2C-GFP
Plasmid#166674PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
Wild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCenpf
Plasmid#160956PurposeCenpf shRNA in pMKO.1 retroviral vectorDepositorInsertCenpf shRNA (Cenpf Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-Nprot_GC3opt
Plasmid#157730Purposemammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertNprot_GC3opt (N SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits