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Plasmid#213285PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR55 (GPR55 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH0001
Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)
Plasmid#214885PurposeLentiviral vector encoding TMP-regulatable RfxCas13d-DD fusionDepositorInsertEF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag
Plasmid#51246PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-CDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-BFP
Plasmid#188775PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR2 (CXCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HCTR2-DuET
Plasmid#213311PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCTR2 (HCRTR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD2-DuET
Plasmid#213228PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD2 (DRD2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-DAP5-3XFLAG
Plasmid#127331PurposeExpress 3xFLAG tagged DAP5 protein from AUG start codonDepositorInsertDAP5 (EIF4G2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
HCTR1-DuET
Plasmid#213310PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHCTR1 (HCRTR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry
Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HTR2A-DuET
Plasmid#213321PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR2A (HTR2A Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
hCXCR4-LSSmOrange
Plasmid#110197PurposeEncodes for human CXCR4 coding sequence tagged with the LSS-mOrange FPDepositorInserthCXCR4 (CXCR4 Human)
UseTagsLSSmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…ExpressionMutationPromoterSOX10 Multiple Species Conserved enhancer element…Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-XBP1 mNeonGreen NLS
Plasmid#115971PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen (retroviral vector backbone)DepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseRetroviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_9_003
Plasmid#167349PurposeddPCR gating control for droplets containing either gag+env targets OR tat + env targetsDepositorInsertgag_tat_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_6_002
Plasmid#167348PurposeddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targetsDepositorInsertpol_gag_tat
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Seattle_IPDA_control_29_006
Plasmid#167352PurposeddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targetsDepositorInsertpol_env
UseOtherTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only