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Plasmid#133317Purposeexpresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SFFV-AID-NTERM-GFP
Plasmid#185763PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos15)GFP
Plasmid#89248Purpose[Edit] Expresses GFP with +15 theoretical charge in E.ColiDepositorInsertHis6-(pos15)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo ACTN1-GFP
Plasmid#138293PurposeMammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion proteinDepositorInsertACTN1 (ACTN1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc
Plasmid#24560DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L
Plasmid#52481PurposeExpresses mCherry, EGFP and Synaptophysin-TagRFP-TDepositorInsertsmCherry
EGFP
Synaptophysin-TagRFP-T
UseRabies virusTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-Isce1-eno2:egfp
Plasmid#61394PurposeExpresses GFP in neurons of transgenic zebrafishDepositorInserteno2 regulatory element
UseZebrafish expressionTagsExpressionMutationPromotereno2Available sinceDec. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAF0432 Ef1a-Dn29-2a-EGFP
Plasmid#193459PurposeExpression of RecombinaseDepositorInsertDn29
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK2 (K57A)
Plasmid#197114PurposeExpression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cellsDepositorInsertTAOK2 (K57A) (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PITX3-eGFP-PGK-Puro
Plasmid#31942DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H3C2
Plasmid#207783PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorArticleInsertH3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKLV2-EF1a-GFP ArgSen
Plasmid#184695PurposeLentiviral transfer plasmid for expression of GFP ArgSenDepositorInsertGFP ArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-eGFP_IDG-K
Plasmid#135264PurposeGateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-eGFP
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LOx-IRES-EGFP
Plasmid#166877PurposeExpress the bacterial lactate oxidase (LOx) enzyme in mammalian cellsDepositorInsertLactate oxidase
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-eGFP-dSaCas9-blast
Plasmid#191653PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.DepositorInserteGFP-T2A-dSaCas9
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)-L-GFP
Plasmid#193716PurposeExpression of GFP tagged human kinesin-1 (KIF5B aa1-560)DepositorInserthsKIF5B(1-560)-L-GFP (KIF5B Synthetic, Human)
UseTagsEGFPExpressionMammalianMutationhsKIF5B(aa1-560);PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniCMV eGFP hPGK BSD
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only