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Plasmid#192276PurposeEncodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoterDepositorInsertCas12m ΔZF
UseCRISPRTagsExpressionBacterialMutationH459A, C552APromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2
Plasmid#196860PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2DepositorInsertNedd1-mOrange2 (Nedd1 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP
Plasmid#196874PurposeNeuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmidsDepositorInsertLynGFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP
Plasmid#196876PurposeNeuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmidsDepositorInsertLynEGFP
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196883PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]
Plasmid#196884PurposeDual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-Lyn-GFP-bGH
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196886PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ATG9A-N
Plasmid#198530PurposeExpression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS_phleo-Tps1/Gsy2
Plasmid#196612PurposeEncoding guide RNAs for the knock out of TPS1 and GSY2 genesDepositorUseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG
Plasmid#148828PurposeMammalian Expression of HsNot9-shRNA2resDepositorInsertHsNot9-shRNA2res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG
Plasmid#148829PurposeMammalian Expression of HsNot9-V181E-shRNA2resDepositorInsertHsNot9-V181E-shRNA2res (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF
Plasmid#148731PurposeBacterial Expression of HsNot10_25-707-HsNot11_257-498DepositorInsertHsNot10_25-707-HsNot11_257-498 (CNOT10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNot9_19-285_AF
Plasmid#148733PurposeBacterial Expression of HsNot9_19-285DepositorInsertHsNot9_19-285 (CNOT9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorUseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_C4a
Plasmid#194973PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_C4d
Plasmid#194975PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mEGFP-Gphn_P1
Plasmid#194978PurposeAAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoterDepositorInsertmEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
UseAAVTagsmEGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-mScarlet-Gphn_P1
Plasmid#194972PurposeAAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoterDepositorInsertmScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)
UseAAVTagsmScarletExpressionMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only