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Plasmid#31138DepositorInsertADAM7 (ADAM7 Human)
UseTagsFLAGExpressionMammalianMutationHistidine 243 changed to TyrosinePromoterAvailable sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-pA-Hia5
Plasmid#222303PurposePlasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertpA-Hia5
UseTags6His and protein AExpressionBacterialMutationcodon optimized for bacterial expressionPromoterrhaBADAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hCB2R
Plasmid#179322PurposeExpress human cannabinoid 2 receptor (GFP tagged) in mammalian cellsDepositorInserthuman cannabinoid 2 receptor (CNR2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKb-TnpB1
Plasmid#215333PurposeISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI siteDepositorInsertRice codon optimized ISDra2 TnpB
UseTagsNot applicableExpressionPlantMutationPromoterRice ubiquitin promoter for TnpB and OsU3 promote…Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1-49535-sgFmr1_CGG5-2
Plasmid#222964PurposeTargeting FMR1 5' UTR for CGG deletionDepositorInsertFMR1 sgRNA (FMR1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCX-CD28 Y189F
Plasmid#223641PurposeRetroviral expression of mouse CD28 (Y189F) in mammalian cellsDepositorInsertCD28 (Cd28 Mouse)
UseRetroviral; mammalian expressionTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCX-CD28 WT
Plasmid#223640PurposeRetroviral expression of mouse CD28 in mammalian cellsDepositorInsertCD28 (Cd28 Mouse)
UseRetroviral; mammalian expressionTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-mTFP1
Plasmid#223638PurposeLentiviral expression of human CD28 with mTFP1 tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsmTFP1ExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-mGFP-SNX9
Plasmid#223632PurposeLentiviral expression of human SNX9 with mGFP tag in mammalian cellsDepositorInsertSNX9 (SNX9 Human)
UseLentiviral; mammalian expressionTagsmGFPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-ALFA-CD28
Plasmid#223630PurposeLentiviral expression of human CD28 with ALFA tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsALFA tagExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28(Y209F)
Plasmid#223627PurposeLentiviral expression of human CD28 (Y209F) in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsExpressionMutationY209FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28(Y191F)
Plasmid#223625PurposeLentiviral expression of human CD28 (Y191F) in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsExpressionMutationY191FPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28
Plasmid#223622PurposeLentiviral expression of human CD28 with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003ExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-LgBiT
Plasmid#223616PurposeLentiviral expression of human CD28 with LgBiT tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsLgBiTExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD28-CLIP
Plasmid#223610PurposeLentiviral expression of human CD28 with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-CD28
Plasmid#223600PurposeLentiviral expression of human CD28 with CLIP tag in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)(W33A)
Plasmid#221761PurposeStable expression of GFP-CERT(PHD)(W33A) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116, mutated 33W to 33APromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-Btk1(PHD)-GFP
Plasmid#221756PurposeStable expression of Btk1(PHD)-GFP in mammalian cellsDepositorInsertBtk (BTK Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–177PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only