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Plasmid#86003Purposehomology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassetteDepositorInserteGFP_T2A_puro_dTK
UsePiggybac exciseableTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PAX6 CKO 2/7 Donor plasmid
Plasmid#68467Purposedonor plasmid for targeting PAX6 locusDepositorInsertFRT flanked exon4 of PAX6 gene (PAX6 Human)
UseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-MYC-ITFG2
Plasmid#87046Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseTagsMYCExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
plc242-GFP-ITFG2
Plasmid#87049Purposemammalian expression of ITFG2DepositorInsertITFG2 (ITFG2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
ND4 left TALE–G1333 DddAtox C
Plasmid#158092Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertSOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
mADAM10 deltaMP in pQCXIP cFLAG
Plasmid#19138DepositorInsertADAM10 (Adam10 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationdelta 56-457PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
ND4 right TALE–G1333 DddAtox N
Plasmid#158096Purposeexpresses the right-side ND4 TALE–DddAtox half in mammalian cellsDepositorInsertCOX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
G1333 DddAtox-C–SaKKH-Cas9(D10A)
Plasmid#157838Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgAtm/Cre
Plasmid#89643PurposeExpresses an Atm-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Atm (Atm Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-N–SaKKH-Cas9(D10A)
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE9aP
Plasmid#12434DepositorUseRetroviralTagsHAExpressionMammalianMutationAML1-ETO9a cDNA as in t(8;21) patientsPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
PAX6 sgRNA7
Plasmid#68466Purposetargeting PAX6 geneDepositorInsertsgRNA targeting PAX6 (PAX6 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
KG371-GFP-mios
Plasmid#87052Purposemammalian expression of miosDepositorInsertmios (MIOS Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa12_807
Plasmid#49663DepositorInsertECF12_807
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3_Z
Plasmid#148151PurposeMammalian Expression of Hs4EBP3DepositorInsertHs4EBP3 (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only