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Plasmid#103583PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-505-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-505-3p target (MIR505 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1307-3p
Plasmid#103214PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1307-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1307-3p target (MIR1307 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30c-1-3p
Plasmid#103413PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30c-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30c-1-3p target (MIR30C1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-27a-3p
Plasmid#103381PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-27a-3p target (MIR27A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-3p
Plasmid#103273PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-3p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285/-169
Plasmid#31052DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-285 to -1 of P2 HNF4A promoter with mutation -16…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285/-136
Plasmid#31051DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutation-285 to -1 of P2 HNF4A promoter with mutation -13…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-23a-5p
Plasmid#103372PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-23a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-23a-5p target (MIR23A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL
Plasmid#58414PurposeExpresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHA internalExpressionMammalianMutationCysteines 71, 72, and 105 to Alanine; The sequenc…PromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-25-5p
Plasmid#103376PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-25-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-25-5p target (MIR25 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7e-5p
Plasmid#103153PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7e-5p target (MIRLET7E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7e-3p
Plasmid#103152PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7e-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7e-3p target (MIRLET7E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-200b-3p
Plasmid#103330PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-200b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-200b-3p target (MIR200B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-132-3p
Plasmid#103219PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-132-3p target (MIR132 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2c-K660E
Plasmid#45706DepositorInsertFGFR2 IIIc (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationK660EPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) E209A (pGEX2T(TEV))
Plasmid#80640PurposeResidues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationChanged Glutamate 209 to alaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBp-FGFR2c-T787K
Plasmid#45710DepositorInsertFGFR2 IIIc (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationT787KPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-T787K
Plasmid#45709DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationT787KPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-E471Q
Plasmid#45703DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationE471QPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only