Addgene

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Human MAC-1 alpha

Plasmid

#8631

Depositor

Timothy Springer

Article

Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

Insert

MAC-1 alpha (ITGAM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RRV

Plasmid

#188543

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RRV

Use

CRISPR; Gateway compatible lbcas12a-rrv entry clo…

Tags

NLS

Expression

Plant

Mutation

G146R, D156R, R182V

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB512

Plasmid

#74854

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2APDGFB

Plasmid

#136409

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

BbsI restriction site in the PDGFB sequence (Glu1…

Promoter

U6, PDK

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB511

Plasmid

#74853

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB406

Plasmid

#74800

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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2xFKBP-mCh-KDEL

Plasmid

#173010

Purpose

FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

2xFKBP-mCh-KDEL

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB414

Plasmid

#74808

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB420

Plasmid

#74814

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB514

Plasmid

#74856

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLLF1(gp350)

Plasmid

#195837

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF1 (BLLF1 Epstein-Barr virus)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB517

Plasmid

#74859

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB442

Plasmid

#74828

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDG461

Plasmid

#100902

Purpose

SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-EGFP

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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