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Plasmid#122676PurposeExpresses mouse PCSK7 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 7 (Pcsk7), transcript variant 2 (Pcsk7 Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)
Plasmid#140226PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistanceDepositorInsertLuciferase + crIFNg
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-eA3Amax-T31A-NG-BlastR
Plasmid#153000PurposeC-to-T/G/A base editorDepositorInserteA3A-BE4 T31A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
UseTagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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EK0290 SFFV-mCherry-SG(s70587-AND-s17d9b)SG-EGFP (FLP-IN)
Plasmid#191143PurposeExpression of Sensor for (1 AND 2) "EK0208 AND EK0211" in mammalian cells; mCherry marker, EGFP output.DepositorInsertmCherry:s70587-AND-s17d9b:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC17orf89-3
Plasmid#86136PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C17orf89DepositorInsertsgRNA 3 targeting C17orf89 (NDUFAF8 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgC17orf89-1
Plasmid#86137PurposeLentiviral vector expressing Cas9 and an sgRNA targeting C17orf89DepositorInsertsgRNA 1 targeting C17orf89 (NDUFAF8 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-HA-shift-IRES-GFP(KaganE21)
Plasmid#52004PurposeExpresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP markerDepositorInsertMAVS-Shift
UseRetroviralTagsHAExpressionMammalianMutationA 2 nucleotide insertion was introduced between t…PromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pN006
Plasmid#188470PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)DepositorInsertAsCas12a
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pN256
Plasmid#188472PurposescDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFPDepositorInserteGFP-WPRE-DAISY-4T
UseTagsExpressionMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011
Plasmid#92320PurposeN-term 1-1011 of HCF-1 proteinDepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-1011PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-JNKKTRmRuby2
Plasmid#59148PurposeORF encoding JNK KTR mRuby2 flanked by Gateway sequencesDepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseTagsmRuby2ExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vclb
Plasmid#185411PurposeGFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin b (vclb Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-GFP
Plasmid#52739PurposeRetroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
Plasmid#216170PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd
Plasmid#216172PurposeExpress the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- Control- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only