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Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046763
Plasmid#145606PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorInsertMANF (MANF Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
cPE
Plasmid#183549PurposeExpresses compact Prime Editor in mammalian cellsDepositorInsertSpCas9 nickase (H840A), Reverse Transcriptase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH3185
Plasmid#132367PurposePrgef-1 GFP::DAF-16a unc-54 3' UTR C.elegans pan-neural expression of isoform a of DAF-16 fused to GFPDepositorInsertDAF-16a (daf-16 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR-SUMO1
Plasmid#114388PurposepDONR vector with SUMO1 geneDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionMammalianMutationQ29H compared with NCBI reference NP_003343.1PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP-T43NTR-TrxA-H10
Plasmid#121677PurposeBacterial expression of neurotensin receptor fusion proteinDepositorInsertneurotensin receptor (Ntsr1 Rat)
UseTagsTrxA-H10ExpressionBacterialMutationdeleted amino acids 1-42PromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gst (Ser)
Plasmid#109354PurposeGsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)DepositorInsertGst chimera
UseTagsNoneExpressionMammalianMutationChanged Cysteine at position 391 to Serine (Pertu…PromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB-II-I-GFP alpha isoform
Plasmid#22146DepositorInsertTFII-I (GTF2I Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS Wnt-3aHA
Plasmid#17374DepositorInsertWnt-3aHA (Wnt3a Mouse)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_PDGFRB
Plasmid#183314PurposeAll-in-One CRISPRko system with a guide RNA that targets PDGFRB geneDepositorInsertPDGFRB
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-ING2
Plasmid#13295DepositorInsertING2 (INGX Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMC-Ura-Cen-lvl1
Plasmid#176182Purposepreassembled yeast MoClo level-1 backbone with GFP dropout and URA3 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MCM6-N-22
Plasmid#54172PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertMCM6
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 SWS cone opsin
Plasmid#109363PurposeHuman SWS cone opsin with 1D4 tagDepositorInsertSWS cone opsin (OPN1SW Human)
UseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec16B
Plasmid#66607PurposeExpresses Sec16B as a GFP fusionDepositorInsertSec16B (SEC16B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only