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AAVS1-Puro XLone-CCND2

Plasmid

#179845

Purpose

donor plasmid for inducible expression of CCND2 in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA3

Plasmid

#220563

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA3 (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA4

Plasmid

#220564

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA4 (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_H6_His

Plasmid

#201719

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_H6 (S )

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSD090A

Plasmid

#218132

Purpose

Hybrid circuit displaying mmCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

Crbn (Crbn Mouse)
luxAB

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD138A

Plasmid

#218130

Purpose

Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Use

Tags

scP22

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD094B

Plasmid

#218131

Purpose

Hybrid circuit displaying mmCRBN bait and driving expression of gIII

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

Crbn (Crbn Mouse)
M13 pIII

Use

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

Plasmid

#209783

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Tags

HA

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN

Plasmid

#209786

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN

Use

AAV

Tags

Expression

Mutation

Promoter

TNT4

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Amt LDHA

Plasmid

#209408

Purpose

Retroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Kaymak et al Cell Metab. 2022 Sep 6;34(9):1298-1311.e

Insert

LdhA shRNA (Ldha Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318; A341)

Plasmid

#204194

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318; L340)

Plasmid

#204195

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318; Gly to Leu 340

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318;A341; A1171)

Plasmid

#204196

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341,1171

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A341; A1171)

Plasmid

#204197

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1a (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 341, 1171

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHAND1-T2A-dTom-PGK-Neo

Plasmid

#209838

Purpose

Tomato reporter plasmid targeting the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

Insert

HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-gRNA

Plasmid

#215547

Purpose

gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M (B2M Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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