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Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ
Plasmid#153036PurposeFor CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos4a-N1
Plasmid#54811PurposeLocalization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4aExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIV URA PCP-NLS-GFPEnvy
Plasmid#189941PurposeSingle-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiaeDepositorInsertPCP-NLS-GFPEnvy
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHA-mLRH-1
Plasmid#16342DepositorInsertLRH-1 (Nr5a2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
Plasmid#74010PurposeTissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoterDepositorInserts5xUAS
Cas9
Cre
U6 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(4S-9) pET29b
Plasmid#75146Purposereprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationN98D S102C A104V A118T F122A K134R F144L I182V E1…PromoterT7Available sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-tdTomato
Plasmid#104067PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomatoDepositorInsertCRY2low-tdTomato
UseTagsExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
T5-sgRNA
Plasmid#113655PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter T5 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterT5Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE_tau-LacZ::tTA-H100Y
Plasmid#34927DepositorInsertsUseMouse TargetingTagsLacZExpressionMammalianMutationMade doxycycline insensitive by introduction of a…PromotertetOAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-IodoY
Plasmid#113645PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.DepositorInsertIodoYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMM131
Plasmid#127214PurposeBeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDSDepositorInsertLuc, AtSTM, sgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC12
Plasmid#104786PurposepNB184-Cas9 entry cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA_Tet3
Plasmid#85740PurposeshRNA against mouse Tet3DepositorInsertTet3 (Tet3 Mouse)
UseAAVTagsEYFPExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOLA banana sensor sfGFP
Plasmid#107367PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNADepositorInsertbanana-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbV-EGFP
Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only