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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#2

Plasmid

#174153

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-5

Plasmid

#118023

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-5 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#1

Plasmid

#174152

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPHAGE-CR-NENF-C-TAP

Plasmid

#128511

Purpose

Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

NENF (NENF Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Synonymous silent mutations to F81, Y82, G83 and …

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-PR.CC9

Plasmid

#192932

Purpose

pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

Plasmid

#157951

Purpose

Lentiviral expression of HBG site 1 sgRNA

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

Plasmid

#184556

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

Plasmid

#184557

Purpose

Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

Depositor

Sandeep Prabhu

Article

Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

Insert

Kirsten rat sarcoma virus (Kras Mouse)

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209061

Purpose

Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

KDM6A

Use

Gateway vector to be used for lr reaction

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAd-PR.Cre

Plasmid

#192936

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1 and Cre

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Trp53 (Trp53 Mouse)
Rb1 (Rb1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-PRL.Cre

Plasmid

#192934

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53, Rb1 and Rbl2 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1, Rbl2 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pShuttle-PR.Cre

Plasmid

#192933

Purpose

pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAd-PR.CC9

Plasmid

#192935

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53 and Rb1, Cre and Cas9

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Trp53 (Trp53 Mouse)
Rb1 (Rb1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307_CR-PDHA2S293A

Plasmid

#115204

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307_CR-PDHA2

Plasmid

#115199

Purpose

Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307_CR-PDHA2S291A

Plasmid

#115203

Purpose

Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

c.321C>T (silent when translated to protein, c…

Promoter

EF1alpha

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1-alpha promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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