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Plasmid#62979PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 1-446 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS19
Plasmid#158788PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans ASH neuronsDepositorInsertpsra-6::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS16
Plasmid#158786PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)DepositorInsertPntc-1::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125a-3p
Plasmid#103187PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125a-3p target (MIR125A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-335-3p
Plasmid#103446PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-335-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-335-3p target (MIR331 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-W290C
Plasmid#45700DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationW290CPromoterAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-495-5p
Plasmid#103569PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-495-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-495-5p target (MIR495 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-324-3p
Plasmid#103435PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-324-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-324-3p target (MIR324 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-324-5p
Plasmid#103436PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-324-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-324-5p target (MIR324 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-24-1-5p
Plasmid#103374PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-24-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-24-1-5p target (MIR24-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
Plasmid#72608PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine37 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ADAM7 (wt)
Plasmid#31139DepositorInsertADAM7 (ADAM7 Human)
UseTagsFLAGExpressionMammalianMutationwtPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb7-PIR-SH2
Plasmid#46443DepositorInsertGrb7 (GRB7 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 334-532PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat2-SH2
Plasmid#46512DepositorInsertStat2 (STAT2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 566-681PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
J23112-mRFP1-331Bb
Plasmid#78278PurposemRFP1 behind constitutive promoter J23112 in pSEVA331Bb backboneDepositorInsertJ23112-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23103-mRFP1-331Bb
Plasmid#78273PurposemRFP1 behind constitutive promoter J23103 in pSEVA331Bb backboneDepositorInsertJ23103-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23105-mRFP1-331Bb
Plasmid#78275PurposemRFP1 behind constitutive promoter J23105 in pSEVA331Bb backboneDepositorInsertJ23105-mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P2
Plasmid#64786PurposeLuciferase expression driven by miR-34a promoter P2DepositorInsertmiR-34a promoter P2 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX HSH2-SH2
Plasmid#46446DepositorInsertHSH2 (HSH2D Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-134PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only