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CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

Plasmid

#135460

Purpose

Mammalian expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape

Use

Tags

HA, flag,

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2 K52R

Plasmid

#118298

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 K52R (Mapk1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

K52R

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

myo-2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle-HA-FLAG-TIN2

Plasmid

#35025

Depositor

Sheila Stewart

Article

Nittis et al Mol Cell Proteomics. 2010 Jun;9(6):1144-

Insert

TIN2 (TINF2 Human)

Use

Adenoviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
BcLOV-mCh-SOS

Plasmid

#208279

Purpose

Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCh-SOS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pECFP

Plasmid

#70106

Purpose

mammalian expression of hSERT with C-terminal CFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pU6-gRNA

Plasmid

#53062

Purpose

Transcription of guide RNA (gRNA) in wheat cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

wheat U6 promoter - gRNA

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

wheat U6

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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LbCas12a

Plasmid

#188493

Purpose

Expresses FLAG tagged LbCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA4 (MSW#517)

Plasmid

#208070

Purpose

Expression of GFP-tagged hsRPA4 in human cells

Depositor

Marc Wold

Article

Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

Insert

RPA4 (RPA4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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OPN(-213)-luc

Plasmid

#31107

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

promoter region -213 to +87

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_miRFP703

Plasmid

#134985

Purpose

The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

miRFP703

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMTP140 (pBAD322G_TniQ-Cascade(Tn6900))

Plasmid

#164262

Purpose

Vector expressing the TniQ, Cas8/5, Cas7, and Cas6 proteins from the Tn6900 element with an arabinose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

Codon-optimized tniQ-cas8/5-cas7-cas6 operon from Tn6900 from A. salmonicida S44 pS44-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf9b

Plasmid

#158378

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9b

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCOLA kiwi sensor sfGFP

Plasmid

#107368

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

kiwi-detecting toehold + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA5-Halo

Plasmid

#175326

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA5 (HSPA5 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Use

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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