-
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
-
MAC-N-GFP
Plasmid#172974PurposeMAC-tagged GFP expressionDepositorInsertGFP
UseTagsMAC-tagExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCiPS-NIFV
Plasmid#110807PurposeNon-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cellsDepositorInsertPol
UseTagsExpressionMammalianMutation2 mutations in the DDE catalyic core motif of the…PromoterCMVAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS
Plasmid#199347PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNC Wnt-3aHA
Plasmid#18030DepositorInsertWnt-3a (Wnt3a Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MCM6-C-19
Plasmid#54171PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertMCM6
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
LbdCas12a KRAB
Plasmid#188503PurposeExpresses FLAG tagged LbdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6640 MSCV-P C-FlagHA 16E7-Kozak
Plasmid#35018DepositorInsertHPV16 E7 (E7 HPV 16)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh20.1 GFP
Plasmid#209106PurposeGFP expressing scramble of shRNA targeting Cdh20DepositorInsertscrCdh20.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU3-gRNA
Plasmid#53063PurposeTranscription of guide RNA (gRNA) in rice cellsDepositorInsertOsU3 promoter + gRNA scaffold
UseCRISPR; Plant expressionTagsExpressionMutationPromoterrice U3Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-3HA
Plasmid#41609DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, TRP1 reverse orientation, and yeast ADH1 te…ExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)ExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA70 (MSW#473)
Plasmid#208067PurposeExpression of GFP-tagged hsRPA1 in human cellsDepositorInsertRPA1 (RPA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP1-Halo
Plasmid#175300PurposeProduction of HaloTag fusion proteinDepositorInsertIGF2BP1 (IGF2BP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only