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Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only
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pCS2 Flag Smad3 EPSM V179T
Plasmid#26998DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179V S204A S208A S213A; V179TPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAV104
Plasmid#63194PurposepRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CIBN-EGFP-KRasCT
Plasmid#178527PurposeExpresses CIBN-EGFP-KRasCT in mammalian cellsDepositorInsertCIBN-EGFP-KRasCT
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI Flag Smad3 LC EPSM
Plasmid#27038DepositorInsertSmad3 (SMAD3 Human)
UseTagsFlagExpressionMammalianMutationaa 133-425; T179V S204A S208A S213APromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Tmem214
Plasmid#175153PurposeLentiviral expression of V5-tagged mouse Tmem214DepositorInsertTmem214 (Tmem214 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR(K30/32L)
Plasmid#181831PurposePhospholipid binding-deficient Lyn-PlcR negative-control plasmid.DepositorInsertLyn-PlcR (K30L,K32L)
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationLysines 30 and 32 in PH domain mutated to leucine.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorInsertSmpd4 (Smpd4 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI Flag Smad3 NL EPSM
Plasmid#27006DepositorInsertSmad3 (SMAD3 Human)
UseTagsFlagExpressionMammalianMutationaa 1-226; T179V S204A S208A S213APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mRuby2-roGFP1
Plasmid#107219PurposeMammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.DepositorInsertmRuby2-roGFP1
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-roGFP1-mApple
Plasmid#107218PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.DepositorInsertroGFP1-mApple
UseTags4xCox8ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-mouse Plaat3 (N157stop)
Plasmid#168771PurposeExpresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cellsDepositorInsertHrasls3 (Plaat3 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-iRFP
Plasmid#175830PurposeExpress iRFP with microtubule-targeting sequence in mammalian cellsDepositorInsertiRFP with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonTagsExpressionBacterialMutationnoPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42
Plasmid#153525PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#42DepositorInsertSb#42
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
UseTagsStrep II tag on Cas1ExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only