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We narrowed to 61,488 results for: promoter

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Showing: 761 - 780 of 61488 results

  3. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    Encodes anti-HA scFv (anti-HA frankenbody) fused with mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    129593-plasmid-map-sequence-id-254271

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  5. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60231-plasmid-map-sequence-id-417804

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  6. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

    Plasmid
    #100854
    Purpose
    AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Has service
    AAV1, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    jRGECO1a
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100854-plasmid-map-sequence-id-466906

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  7. pX330 Pten

    Plasmid
    #59909
    Purpose
    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Pten (Pten Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59909-plasmid-map-sequence-id-123315

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  9. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187242-plasmid-map-sequence-id-374822

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  10. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Publication
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111905-plasmid-map-sequence-id-512037

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  15. MLM3613

    Plasmid
    #42251
    Purpose
    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters
    Depositor
    Keith Joung
    Publication
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptococcus pyogenes Cas9
    Use
    CRISPR; Zebrafish expression
    Promoter
    CMV
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42251-plasmid-map-sequence-id-59208

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  17. pAAV.Syn.GCaMP6s.WPRE.SV40

    Plasmid
    #100843
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100843-plasmid-map-sequence-id-447138

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  18. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  19. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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Showing: 761 - 780 of 61488 results