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pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacI

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS12

Plasmid

#192468

Purpose

Bacterial expression of RvCAHS12

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET11b-HA2

Plasmid

#165992

Purpose

Influenza HA ministem with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

HA2

Use

Tags

Foldon and HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET24c(+)-EFTu

Plasmid

#31172

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Tu (tufB E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Use

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Use

Tags

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETM33_CALM1_EF-hand

Plasmid

#178468

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CALM1_EF-hand (CALM1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH2

Plasmid

#204230

Purpose

Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH2 (MDH2 Human)

Use

Tags

6X His

Expression

Bacterial

Mutation

Does not include transit peptide and includes a M…

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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SIRT5-pET15b

Plasmid

#81030

Purpose

Bacterial expression of Human WT SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a FKBP12-V5

Plasmid

#194117

Purpose

Expresses FKBP12 protein with V5 tag

Depositor

Jun Liu

Insert

FKBP1A (FKBP1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-HADH2

Plasmid

#67863

Purpose

bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

HADH2 (HSD17B10 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet-LahT150

Plasmid

#166785

Purpose

Cleavage of double glycine motif in peptides to remove the leader sequence

Depositor

Wilfred van der Donk

Article

Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

Insert

LahT150

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K48C

Plasmid

#18897

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin-K48C (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Lysine 48 is replaced with a Cysteine residue

Promoter

Available since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pET-3c-rhVTN-NC

Plasmid

#30226

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 Apr 10. ():.

Insert

rhVitronectin with N and C terminus cleavages (VTN Human)

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids: 399-478, 20-62

Promoter

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

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