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Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST eIF2beta
Plasmid#45900DepositorInserteIF2S2 (Eif2s2 Mouse)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSV HA eIF2beta WT
Plasmid#45898DepositorInsertEif2s2 (Eif2s2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom4.2
Plasmid#87347PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 4.2 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter4.2 kbp proximal promoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSA
Plasmid#195207PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340APromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ7-DadR/DadS-Pdadh-LacZ
Plasmid#191622PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadSDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9.1-HcdR-Phcdh-LacZ
Plasmid#191629PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564A
Plasmid#191978PurposeTo produce mutants mimicking dephosphorylated Mfn1DepositorInsertMfn1 T564A (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564APromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-Mfn1-T564D
Plasmid#191979PurposeTo produce mutants mimicking phosphorylated Mfn1DepositorInsertMfn1 T564D (Mfn1 Mouse)
UseTagsExpressionMammalianMutationT564DPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ4
Plasmid#126407PurposeExpression of Smoothened NQ4 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ4PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 mSmoothened NQ3
Plasmid#126406PurposeExpression of Smoothened NQ3 in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationSmo glycosylation site NQ3PromoterAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-Cx43-FS
Plasmid#65440PurposeCloning, T7 RNA expressionDepositorInsertCx43 (GJA1 Human)
UseT7 in vitro rna productionTagsExpressionBacterialMutationFrameshift (FS) mutation 35delAPromoterT7Available sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-D92N
Plasmid#58412PurposeExpresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationApartate 92 to AsparaginePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-N2A
Plasmid#58410PurposeExpresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationAsparagine 2 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-S38N
Plasmid#58411PurposeExpresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationSerine 38 to AsparaginePromoterCMV IEAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only